发明名称 |
PRODUCTION OF LOW FUCOSE ANTIBODIES IN H4-II-E RAT CELLS |
摘要 |
<p>The invention concerns the field of cell culture technology. It specifically concerns a rat hepatoma cell, preferably a H4-II-E rat hepatoma cell, carrying a DNA encoding an antibody or Fc-fusion protein and having low fucosylation activity for adding fucose to glycosidic structures such as biantennary glycans, e.g. N-acetylglucosamine. The invention furthermore concerns a method for producing low fucose glycoproteins especially antibodies or Fc-fusion proteins in rat hepatoma cells, preferably in H4-II-E rat hepatoma cells. It further concerns the identification and generation of new host cell lines which are capable of synthetizing glycoproteins with beneficial properties, improving the therapeutic efficacy and/or serum half-life of the product compared to products from commonly used host cell lines.</p> |
申请公布号 |
EP2622066(A1) |
申请公布日期 |
2013.08.07 |
申请号 |
EP20110763630 |
申请日期 |
2011.09.23 |
申请人 |
BOEHRINGER INGELHEIM INTERNATIONAL GMBH |
发明人 |
ELLWANGER, KRISTINA;FLORIN, LORE;KAUFMANN, HITTO |
分类号 |
C12N5/09;A61K39/395;C12N5/16 |
主分类号 |
C12N5/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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