发明名称 |
INFECTIOUS CDNA CLONES OF FOOT-AND-MOUTH DISEASE VIRUS OF TYPE O AND THE COMPLETE SEQUENCES OF THE CLONES |
摘要 |
<p>PURPOSE: A cDNA clone is provided to acquire basic knowledge related to replication, expression, and infection of foot-and-mouth disease virus, and to be used vaccine and diagnostic materials, thereby resolving problems of existing inactivated vaccines. CONSTITUTION: A cDNA clone is prepared using foot-and-mouth disease virus with a base sequence of sequence number 1. The clone is prepared using foot-and-mouth disease virus with a base sequence selected from a group consisted of base sequences of sequence numbers 2, 3, 4, and 5. The clone identifies replication and expression difference of a replicon by manipulating untranslated region (UTR) or T7 promoter site of the clone with a base sequence of sequence number 5. In the clone, G is added at 644nt G and C is substituted with T at 9602nt in T7 promoter. A replicon clone has a base sequence of sequence number 6. [Reference numerals] (AA,BB,CC) Foot-and-mouth disease; (DD) Prepare linear DNA (Nsi cut); (EE) Confirm RNA quantification & integrity; (FF) Confirm virus production; (HH) Acquire infectious foot-and-mouse disease virus or express a non-infectious foot-and-mouth virus-like particle and protein</p> |
申请公布号 |
KR20130078265(A) |
申请公布日期 |
2013.07.10 |
申请号 |
KR20110147110 |
申请日期 |
2011.12.30 |
申请人 |
REPUBLIC OF KOREA(MANAGEMENT:ANIMAL AND PLANT QUARANTINE AGENCY) |
发明人 |
LEE, KWANG NYEONG;KIM, SU MI;PARK, JONG HYEON;KO, YOUNG JOON;LEE, HYANG SIM;CHO, IN SOO;LEE, SEO YONG |
分类号 |
C12N15/33;C12N15/86 |
主分类号 |
C12N15/33 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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