摘要 |
PURPOSE: A method for isolating, concentrating, and detecting targeting-gold magnetic nanoparticles and gold magnetic nanoparticle-based microorganisms in one-step is provided to simultaneously detect target microorganisms in a sample. CONSTITUTION: A method for isolating, concentrating, and detecting microorganisms using gold magnetic nanoparticles comprises: a step of contacting a sample targeting-gold magnetic nanoparticles comprising a core, a shell, and a targeting moiety; and a step of applying the resultant to a column. The magnet is applied by a permanent magnet or electromagnet. The targeting moiety is an antibody or a fragment thereof. |