摘要 |
<P>PROBLEM TO BE SOLVED: To develop a culture method/system which enables separation and culture of a cell in a completely closed system. <P>SOLUTION: A culture method, a culture system and a culture apparatus are provided, in all of which a body fluid containing a blood cell is mixed with an erythrocyte-aggregating agent in a closed system in an aseptic manner, then allowing the resultant mixture to stand to thereby precipitate an erythrocyte, and then injecting a lymphocyte-containing buffy coat layer into a closed culture apparatus in a closed manner, thereby culturing the blood cell, in order to carry out all including separation to culture of the cell in the completely closed system. <P>COPYRIGHT: (C)2013,JPO&INPIT |