发明名称 |
METHODS AND KITS FOR DETERMINING PROTEIN-BOUND BIOMARKERS |
摘要 |
<p>The invention relates to the field of diagnostic methods, in particular to detection of biological molecules using mass spectroscopy (MS). Provided is an automated high-throughput method for quantitating a low molecular weight protein-bound biomarker directly in an isolated biological sample, comprising the steps of: (a) automated prepurification of the sample using an effective amount of an acid protease under conditions that allow for digestion of one or more binding proteins; (b) applying said protease-digested sample onto an on-line SPE column to capture at least part of said biomarker, followed by sequential washing of the solid phase; (c) eluting a fraction comprising said biomarker directly onto a liquid chromatography (LC) column comprising an apolar stationary phase and subjecting it to LC-MS or LC-MS-MS measurements to determine the amount of at least one biomarker; and (d) quantitating the biomarker(s). Also provided are kits for use in such method, for instance in the determination of vitamin D derivatives, testosterone and/or or melatonin.</p> |
申请公布号 |
CA2862260(A1) |
申请公布日期 |
2013.06.06 |
申请号 |
CA20122862260 |
申请日期 |
2012.11.29 |
申请人 |
ACADEMISCH ZIEKENHUIS GRONINGEN;RIJKSUNIVERSITEIT GRONINGEN |
发明人 |
KEMA, IDO PETER;VAN FAASSEN, HERMANNUS JOHANNES ROELOF;MANZ, BERNHARD |
分类号 |
G01N33/538;G01N33/74 |
主分类号 |
G01N33/538 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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