发明名称 |
TSG PRIMER TARGET DETECTION |
摘要 |
The present invention relates to the detection of a target nucleic acid sequence in a real-time manner using a target signal generating primer (TSG primer) having dual interactive labels. The present invention allows for both target amplification and signal amplification by introducing dual interactive labels into a primer used in PCR reactions, ensuring real-time target detection by PCR reactions without the use of complicated oligonucleotides. The present invention could be free from the troublesome matters and shortcomings associated with conventional real-time PCR methods. The present invention allows for successful real-time target detection by using only a labeled primer. Also, the present invention can obtain strong signals indicative of the presence of target nucleic acid sequences in both a liquid phase and solid phase. |
申请公布号 |
EP2516679(A4) |
申请公布日期 |
2013.05.22 |
申请号 |
EP20100839638 |
申请日期 |
2010.03.26 |
申请人 |
SEEGENE, INC. |
发明人 |
CHUN, JONG YOON;HWANG, IN TAEK |
分类号 |
C12Q1/68;C07H21/00;C12N15/11 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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