METHOD FOR PRODUCING TRANSGENIC MISCANTHUS PLANT AND THE PLANT THEREOF
摘要
PURPOSE: A method for producing a transgenic Miscanthus plant is provided to obtain Miscanthus plants with high transformation efficiency using callus. CONSTITUTION: A method for producing a transgenic Miscanthus plant comprises: a step of placing a Miscanthus seed on a callus-inducing medium and inducing Miscanthus callus; a step of co-culturing the induced Miscanthus callus and Agrobacterium tumefaciens containing a foreign gene-inserted plant expression vector; a step of culturing the co-cultured plant cells in a selective medium containing antibiotics; a step of elongating the selected Miscanthus cells in an elongation medium; and a step of inducing rooting of the elongated cells in a medium for inducing rooting.