| 摘要 |
H:/ixp/Intenove n/NRPorbI/DCC/IXP/564 04 _ I.doc-4/10/2013 A method for isolating polynucleotides from a cell-containing sample the method comprising: contacting the sample with a lysis solution and a plurality of binding particles coated in PAMAM (Generation 0), so that the polynucleotides are liberated from the cells and become bound to the PAMAM (Generation 0), thereby creating binding particles bound with polynucleotides and a solution containing residual cellular matter; compacting the binding particles bound with polynucleotides; removing the solution containing residual cellular matter; washing the binding particles; and releasing the polynucleotides from the binding particles. WO 2009/012185 PCU/US2008/069897 I,, IN:~( m*-TIm |
