| 摘要 |
FIELD: biotechnologies.SUBSTANCE: recombinant host cell Pichia pastoris includes a cascade of fucosylation reactions, where ferments make up the specified cascade of reactions. The specified host cell Pichia pastoris includes nucleic acids, which code GDP-mannose-dehydratase (GMD), GDP-ketodesoxymannose-epimerase/GDP-ketodesoxygalactose-reductase (FX), a carrier of GDP-fucose (GFTr) and fused protein, which contains a catalytic domain of ?1,6-fucosyltransferase EC 2,4.1.68, fused with amino acids 1-36 Mnn2 S.cerevisiae. A hybrid vector includes regulatory elements of DNA, which are functional in the host cell and which are functionally connected with a coding DNA sequence, which codes the fused protein, including amino acids 1-36 Mnn2 S.cerevisiae, fused with the catalytic domain of ?1,6-fucosyltransferase EC 2.4.1.68. The specified vector is used to transform the host cell Pichia pastoris.EFFECT: invention makes it possible to create a host cell Pichia pastoris, which will produce glycoproteins with fucosylated N-glycans.8 cl, 6 dwg, 3 ex |
