METHOD FOR DETERMINATION OF NITROGEN IN OZONISED PERFLUORIDECARBON EMULSION

摘要

FIELD: medicine.SUBSTANCE: invention describes a method for determination of nitrogen in an ozonised perfluoridecarbon emulsion by photometric measurement of iodine recovered by a reaction of nitrogen of the ozonised emulsion and a potassium iodide solution with starch; the determination process is preceded by preparing a reaction solution containing potassium iodide and starch to be used to prepare sample and calibration pre-solutions containing the equal proportions of potassium iodide and starch; the sample pre-solution is added with a specific amount of ozonised perftoran, while the calibration pre-solutions are added with the same amount of non-ozonised perftoran and standard iodine in potassium iodine in the amount required to draw a calibration diagram; the prepared solutions are kept in a dark plate at temperature 20°C; the solutions are measured in a photocolorimeter or a spectrophotometer at wave length 610 nm following the time variations of optical density until the curves of the calibration and sample solutions occurs to match; a calibration diagram is drawn, and nitrogen is calculated in the sample emulsion.EFFECT: invention enables determining nitrogen in aqueous-organic emulsions thereby enhancing its analysis in complex, polycomponent heterogenic media.1 ex, 3 dwg