METHOD FOR DETECTING NUCLEIC ACID USING INTERCALATOR-CONJUGATED METAL NANOPARTICLES

摘要

The present invention relates to a method for detecting nucleic acid using intercalator-conjugated metal nanoparticles. More particularly, the present invention relates to a method which involves applying a sample containing a target nucleic acid to a DNA chip having a substrate with a DNA probe fixed thereon, reacting metal nanoparticles in which intercalators coupled with a double helix nucleic acid are conjugated, and reacting a metal enhancing solution to thereby amplify the sizes of the metal nanoparticles and detect the target nucleic acid using the unaided eye. The method for detecting nucleic acid according to the present invention uses intercalator-conjugated metal nanoparticles, and thus enables detection of nucleic acid using the unaided eye without any other equipment. Therefore, compared with conventional detection methods, the present invention exhibits the effects of reducing analysis costs and the time needed for detection, and enables miniaturization of the size of an apparatus, thereby enabling field diagnosis in a livestock farm, home, or the like.