METHOD FOR PURIFICATION AND IDENTIFICATION OF SPERM CELLS
摘要
The present disclosure describes the isolation, identification and purification of aptamers having sufficiently high affinity and specificity to capture and immobilize intact sperm cells in the presence of female epithelial cells and other non-sperm semen components. The present disclosure also describes affinity-based methods for the detection of sperm cells in test samples, including from forensic sample surrogates consisting of swab eluates containing a mixture of HeLa cells and sperm cells. The present disclosure describes methods for eluting sperm cell samples from swabs; methods for purifying sperm cells and methods for amplification and analysis of male DNA. The affinity-based system described herein is inexpensive, simple to use and easily implemented in forensic laboratories.
申请公布号
WO2012174496(A3)
申请公布日期
2013.01.31
申请号
WO2012US42835
申请日期
2012.06.15
申请人
SOMALOGIC, INC.;SANDERS, GLENN;KATILIUS, EVALDAS;GOLD, LARRY