NON-CYTOTOXIC ALPHA VIRAL CONSTRUCT, CELL LINE AND COMPOSITION AND A METHOD FOR THE ASSESSMENT OF THE EFFECT OF ANTIVIRAL AGENTS.

摘要

The invention relates to the field of molecular biology and comprises constructs based on non-cytotoxic alphaviral mutants capable to replicate in a stable manner ant to be maintained in mammalian cells as well as cell lines capable to replicate said constructs in a stable manner. The invention provides new constructs based on an alphavirus, in particular Chikungunya virus, which are feasible to apply in the creation of expression systems and research for and analysis of antiviral substances due to their non-cytotoxic nature. The invention enables the assessment of drug candidates for the screening of anti-alphaviral substances. Also, the invention can be applied for expressing recombinant proteins in genetic engineering systems.