AXIAL LIGHT LOSS SENSOR SYSTEM FOR FLOW CYTOMETERY

摘要

An axial light loss sensor system, and methods for measuring axial light loss with improved resolution are provided. Aspects of the present invention include an axial light loss sensor positioned along an axis of irradiation to detect axial light loss resulting from a particle passing a light source intersect in a fluid stream, and an obstruction positioned along the axis of irradiation between the light source intersect and the axial light loss sensor. The obstruction is further positioned so as to have an on-axis opaque surface. The obstruction allows for the measurement of a fringe signal in a far-field with respect to the irradiated particle, in order to measure the axial light loss produced by the particle. The systems and methods described herein find use in, for example, flow cytometery.