PRODUCTION DE MOLECULES D'INTERET EN MILIEU SOLIDE

摘要

Preparing molecule of interest by fermentation of solid phase substrate comprises: providing a substrate; potentially reducing the size of the substrate; sterilizing the substrate to dry; incubating the substrate under agitation with a microorganism in suspension; fermenting the substrate in the solid state in a reactor; sterilizing the fermented substrate; recovering the substrate by a volume of water of 0.5-5 times the mass of substrate; collecting the washing liquid; extracting the molecules of interest from the liquid; and optionally drying the molecules of interest. Preparing molecule of interest by fermentation of a solid phase substrate comprises: (a) providing a substrate; (b) potentially reducing the size of the substrate so as to obtain 100% of the particles having an average diameter of less than 1 mm; (c) sterilizing the substrate to dry; (d) incubating the substrate under agitation with a microorganism in suspension at a rate of 1x 10 5>-1x 10 7>spores/g of substrate, preferably 2.5 x 10 5>spores/g of substrate to have a dimension (a w) of 0.950-0.999 m, preferably 0.980-0.995 m; (e) fermenting the substrate in the solid state in a reactor without agitating for 2-8 days, preferably 3-6 days at a temperature of 18-30[deg] C under atmospheric pressure; (f) sterilizing the fermented substrate; (g) recovering the substrate by a volume of water of 0.5-5 times the mass of substrate; (h) collecting the washing liquid; (i) extracting the molecules of interest from the liquid collected in step (h); and optionally (j) drying the molecules of interest.