BIO PROCESS ADDITIVES

摘要

Disclosed is a cell culture medium for mammalian cells, and a corresponding process for the manufacture of a protein by a biosynthetic process in a cell culture system comprising mammalian cells, which process comprises keeping the presence of one or more radical scavenging and/or antioxidative agents selected from the group consisting of sterically hindered nitroxyls, sterically hindered hydroxylamines, or salts or adducts thereof, in the culture medium at a level from 5 to 5000 micromol (sterically hindered nitrogen) per litre of the cell culture system through a part or all of the biosynthetic manufacturing process.