| 摘要 |
PURPOSE: A method for preparing a gold-fluorescence silica nanoparticle composite is provided to ensure excellent optical stability and to be used in a biosensor. CONSTITUTION: A gold-fluorescence silica nanoparticle composite contains: fluorescence nanoparticles; gold nanoparticles deposited on the surface of the fluorescence silica nanoparticle; and gold binding peptides conjugated on the surface of the gold nanoparticles. The fluorescence silica nanoparticles is formed in a sphere having 20-900 nm of average diameter. The fluoresence silica nanoparticles is formed by reaction of silica precursors with Rubpy(tris(2,2-bipyridyl)ruthenium(II)), FITC(fluorescein isothiocyanate), DAPI(4',6-diamidino-2-phenylindole), TRTIC(tetramethylrhodamine-5-(and 6)-isothiocyanate), rhodamine, texas red, alexa fluor 350, 405, 430, 488, 500, 514, 633, 647, 660, 680, 700, cy3, cy5, or cy7. A biosensor contains the gold-fluorescence silica nanoparticle complex.
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