发明名称 |
A BIOSENSOR USING IMPEDIMETRIC REAL-TIME MONITORING |
摘要 |
<p>A method an system is disclosed for the detection and/or allocation of at least one point mutation in target DNA and/or RNA duplexes. The method comprises obtaining a functionalized surface which is coated with probe DNA and/or RNA whereto target DNA and/or RNA duplexes are attached, contacting said functionalized surface to an electrolytic solution having a neutral pH in a flow cell and measuring a first impedance value within said electrolytic solution, and then adding a chemical to the electrolytic solution which is able to achieve denaturation of the target DNA and/or RNA. The method further comprises measuring a second impedance value within the flow cell after completion of the denaturation of the DNA and/or RNA target, and then obtaining a value representative for the impact of the chemical on the impedance of the electrolytic solution. The amount and/or allocation of point mutation(s) within the target DNA and/or RNA is then determined by calculating the denaturation-time constant based on the difference between the first and second impedance value and taking into account the impact of the chemical by third impedance value.</p> |
申请公布号 |
WO2012076350(A1) |
申请公布日期 |
2012.06.14 |
申请号 |
WO2011EP71090 |
申请日期 |
2011.11.25 |
申请人 |
IMEC;UNIVERSITEIT HASSELT;VAN GRINSVEN, BART;DE CEUNINCK, WARD;WAGNER, PATRICK |
发明人 |
VAN GRINSVEN, BART;DE CEUNINCK, WARD;WAGNER, PATRICK |
分类号 |
C12Q1/68;G01N27/02;G01N27/327 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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