| 摘要 |
Disclosed is a nucleic acid amplification method which is based on a new principle and enables to amplify a nucleic acid having a specific nucleotide sequence in a simple manner, within a short time and with efficiency. The nucleic acid amplification method comprises the steps of: (a) obtaining a linear DNA fragment by performing a DNA polymerase elongation reaction by using a template DNA comprising a base sequence to be amplified and a primer pair comprising a primer having a base sequence complementary to a region adjacent to a 3' end of the base sequence to be amplified and a chemically modified 3' end; and (b) performing a strand displacement-type DNA polymerase elongation reaction on a circular single-stranded DNA comprising the base sequence to be amplified and serving as a template, with a 3' end of the linear DNA. fragment obtained in (a) serving as an origin of replication.
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