An apparatus and method to maintain pH within a range conducive for cell growth in a bicarbonate-containing cell culture system without the addition of base. The method relies on the gas transfer characteristics of the bioreactor system to modulate the CO2 transfer to and from the cell culture such that the pH of the cell culture can be maintained within a desired range.
申请公布号
EP2451936(A2)
申请公布日期
2012.05.16
申请号
EP20100730960
申请日期
2010.07.06
申请人
F.HOFFMANN-LA ROCHE AG
发明人
BASKAR, DINESH;HSIUNG, JENNY;LEUNG, WOON-LAM, SUSAN;YUK, INN, H.