发明名称 |
Method for the identification of the clonal source of a restriction fragment |
摘要 |
The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3′end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular markers.
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申请公布号 |
US8178300(B2) |
申请公布日期 |
2012.05.15 |
申请号 |
US20070373220 |
申请日期 |
2007.07.10 |
申请人 |
VAN EIJK MICHAEL JOSEPHUS THERESIA;JESSE TACO PETER;KEYGENE N.V. |
发明人 |
VAN EIJK MICHAEL JOSEPHUS THERESIA;JESSE TACO PETER |
分类号 |
C12Q1/68;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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