发明名称 |
PROCESS FOR PRODUCING PROTEIN CAPABLE OF FORMING INCLUSION BODY |
摘要 |
A process for preparing an inclusion body-forming protein is provided. A nucleic acid fragment consisting of a nucleotide sequence coding for a modified alkaline phosphatase signal peptide (modified APSP) where leucine at the 13th position in the amino acid sequence shown in SEQ ID NO: 1 is substituted with proline and/or alanine at the 21st position is substituted with the other amino acid, downstream of which nucleotide sequence is bound a nucleotide sequence of a gene of a protein of interest, and a process for preparing an inclusion body-forming protein consisting of the following steps: (1) preparing an expression vector in which a nucleic acid fragment is incorporated consisting of a nucleotide sequence coding for a modified signal peptide downstream of which is bound a nucleotide sequence of a gene of a protein of interest, (2) preparing a host cell transformed with the expression vector that produces an inclusion body-forming protein, and (3) purifying the inclusion body-forming protein from culture obtained by culturing the host cell that produces the inclusion body-forming protein. The above other amino acid is selected from the group consisting of aspartic acid, glutamic acid, lysine, histidine, phenylalanine and tyrosine. |
申请公布号 |
EP2363469(A4) |
申请公布日期 |
2012.05.02 |
申请号 |
EP20090822044 |
申请日期 |
2009.10.21 |
申请人 |
THE CHEMO-SERO-THERAPEUTIC RESEARCH INSTITUTE;TEIJIN PHARMA LIMITED |
发明人 |
NAKATAKE, HIROSHI;META, AKIHIRO;SUENAGA, KIYOTAKA;HIRASHIMA, MASAKI;MAEDA, HIROAKI |
分类号 |
C12N15/09;C12N1/21;C12N9/64;C12P21/02 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|