MODIFYING GLYCOPROTEIN PRODUCTION IN PLANTS

摘要

Provided is a method for synthesizing a protein of interest with reduced fucosylation and xylosylation comprising, introducing within a plant, a portion of a plant, or a plant cell, a nucleotide sequence encoding a first nucleotide sequence encoding a hybrid protein, GNT1- GalT, comprising a plant-derived CTS domain (cytoplasmic tail, transmembrane domain, stern region) of N-acetylglucosaminyl transferase (GNT1) fused to a catalytic domain of beta-1 ,4 galactosyltransferase (GalT), the first nucleotide sequence operatively linked with a first regulatory region that is active in the plant, and a second nucleotide sequence for encoding the protein of interest, the second nucleotide sequence operatively linked with a second regulatory region that is active in the plant, and transiently co-expressing the first and second nucleotide sequences, to synthesize the protein of interest comprising less than 10% fucosylation and xylosylation when compared to the same protein of interest produced in a wild-type plant.