发明名称 |
METHOD FOR DETECTION OF MUTATIONS |
摘要 |
A method and a kit for detecting a mutation from a non-mutated sequence of a target polynucleotide in a sample, by using a mismatch binding protein. The method comprises: a) providing non-mutated and mutated target polynucleotide; b) forming duplex of the non-mutated and mutated single strands of the target polynucleotide in a); c) adding a single strand binding protein to the polynucleotide from b); d) incubating the mismatch binding protein with an activating agent; e) adding the incubated mismatch binding protein from d) to the polynucleotide from c), whereby the mismatch binding protein binds to the duplex formed by one non-mutated and one mutated single strand of the target polynucleotide; f) detecting the presence of any mismatch binding protein bound to the target polynucleotide. The kit comprises: a) single strand binding protein, b) activator for mismatch binding protein; c) mismatch binding protein; d) amplification primer(s); e) solid support for binding of target polynucleotide or mismatch binding protein.
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申请公布号 |
WO9745555(A1) |
申请公布日期 |
1997.12.04 |
申请号 |
WO1997SE00839 |
申请日期 |
1997.05.22 |
申请人 |
PHARMACIA BIOTECH AB;HASEBE, MASAHISA;GOTO, MASANORI;TOSU, MARIKO |
发明人 |
HASEBE, MASAHISA;GOTO, MASANORI;TOSU, MARIKO |
分类号 |
C12N15/09;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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