发明名称 ENZYME SOLUTION FOR SEPARATING CELL AND METHOD FOR SEPARATING CELL, AND METHOD FOR SEPARATING PANCREATIC ISLET
摘要 <P>PROBLEM TO BE SOLVED: To provide an enzyme solution for separating cell(s), which can reduce damage on separating a single cell or a mass of cells from a tissue or an organ, to provide a method for separating cell(s) using the enzyme solution, and to provide a method for separating pancreatic islets. <P>SOLUTION: The enzyme solution for separating cell(s) includes a chloride ion channel inhibitor or has chloride ion concentration of 10 mM or lower. By an enzymatic treatment using the enzyme solution for separating cell(s), a single cell or a mass of cells can be separated from a tissue or an organ. When pancreatic islet(s) is especially separated from the pancreas, the enzyme solution for separating cell(s) is injected through the pancreatic duct to digest the pancreas. <P>COPYRIGHT: (C)2012,JPO&INPIT
申请公布号 JP2012029623(A) 申请公布日期 2012.02.16
申请号 JP20100171867 申请日期 2010.07.30
申请人 BIO LINK INC 发明人 HAZAMA AKIHIRO;MIYAKE MASAO;GOTO MANICHI;ANAZAWA TAKAYUKI;SHINOZAKI HISAFUMI
分类号 C12N5/071 主分类号 C12N5/071
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