发明名称 |
Method of producing biologically active polypeptide having insulinotropic activity |
摘要 |
The present invention relates to a method of producing a biologically active polypeptide having insulinotropic activity, the method comprising steps of: (a) transforming a genetically modified host cell that has protease gene knockout, with a polynucleotide vector encoding the polypeptide; and (b) growing the transformed host cell to produce the biologically active polypeptide; and a method of producing a biologically active polypeptide having an N-terminal recognition site His-Gly with insulinotropic activity, the method comprising steps of: (a) transforming a genetically modified Pichia pastoris that has protease gene STE13 knockout, with a polynucleotide vector encoding the polypeptide; and (b) growing the transformed Pichia pastoris to produce the biologically active polypeptide. |
申请公布号 |
US8114632(B2) |
申请公布日期 |
2012.02.14 |
申请号 |
US20070305460 |
申请日期 |
2007.06.20 |
申请人 |
MELARKODE RAMAKRISHNAN;SRIRAM AKUNDI VENKATA;SASTRY KEDARNATH NANJUND;VARADARAJALU LAKSHMI PRABHA;SURYANARAYAN SHRIKUMAR;BIOCON LIMITED |
发明人 |
MELARKODE RAMAKRISHNAN;SRIRAM AKUNDI VENKATA;SASTRY KEDARNATH NANJUND;VARADARAJALU LAKSHMI PRABHA;SURYANARAYAN SHRIKUMAR |
分类号 |
C12N15/09;C12N15/74;C12P21/04;C12P21/06 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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