| 摘要 |
An improved apparatus and method for fluorescence subtraction in Raman spectroscopy, where a narrow band light source and a broad band light source are utilized to stimulate Raman scattering and fluorescence emission from the same subject to produce two Raman/fluorescence spectra. The two light sources, with matched output power, produce similar level of fluorescence emission, yet the Raman scattering signal produced by the broad band light source has much lower spectral intensity than that produced by the narrow band light source. By subtracting the two Raman/fluorescence spectra, the weak Raman signal can be extracted from a strong fluorescence background. |
