| 摘要 |
Disclosed is a transgenic plant comprising a plant chromosomal DNA segment comprising a recombinant polynucleotide flanked by native plant DNA, wherein said polynucleotide provides for expression of at least an NF-YB protein and a marker protein, wherein said NF-YB protein is produced in leaf cells of said plant at a level up to 40 picograms per microgram of total protein in said plant leaf tissue cells, wherein said plant is water deficit stress tolerant, and wherein the harvested yield of said plant is comparable to or enhanced over the yield of a control plant not having said plant chromosomal DNA segment when said plants are grown in water sufficient conditions. Also disclosed is a method for manufacturing non-natural, transgenic seed that can be used to produce a crop of transgenic plants with enhanced water deficit stress tolerance resulting from expression of an NF-YB protein from a plant chromosomal DNA segment comprising a recombinant polynucleotide flanked by native plant DNA that provides for expression of an NF-YB protein in leaf cells of said plant at a level up to 40 picograms per microgram of total protein in said plant leaf tissue cells, wherein said method comprises: (a) screening a population of plants having said plant chromosomal DNA segment and control plants for said enhanced yield when grown under deficit stress or enhanced or comparable yield as compared to the yield for control plants when grown under sufficient water conditions, and (b) selecting from said population one or more plants that exhibit enhanced yield as compared to the yield for control plants under water deficit stressed or enhanced or comparable yield as compared to the yield for control plants when grown under water sufficient conditions.
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