发明名称 |
Multicomponent nucleic acid enzymes and methods for their use |
摘要 |
<p>The present invention relates to Multicomponent Nucleic Acid Enzymes (MNAzymes) and methods for their use. MNAzymes comprise two or more oligonucleotide components which self-assemble in the presence of one or more MNAzyme assembly facilitator molecules to form a catalytically active structure. Compositions for making MNAzymes, and collections of MNAzymes are provided. Also provided are methods for using MNAzymes for the detection, identification and/or quantification of one or more targets. The methods can be practiced in solution-based assays or in assays where one or more reaction components are attached to a support structure. The methods allow for multiplexing the MNAzyme detection to detect multiple targets in a single reaction. Also provided are kits for making the compositions, and for practicing the methods provided herein.</p> |
申请公布号 |
EP2385141(A1) |
申请公布日期 |
2011.11.09 |
申请号 |
EP20110167771 |
申请日期 |
2006.10.06 |
申请人 |
JOHNSON & JOHNSON RESEARCH PTY LIMITED |
发明人 |
MOKANY, ELISA;BIRKETT, DONALD JOHN;TODD, ALISON VELYIAN;DOAN, TRAM BICH |
分类号 |
C12Q1/68;C12N9/00 |
主分类号 |
C12Q1/68 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|