| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a typing method of a tubercle bacillus which can inspect a typing of a tubercle bacillus with sufficient accuracy in a short period of time and simply. <P>SOLUTION: The typing method of a tubercle bacillus is characterized in that a VNTR method and multiplex PCR are combined, a specific primer set is used so that an amplification product in which a molecular weight region shows a different distribution may be acquired, and the size is decided by carrying out electrophoresis with different labeling. Accordingly, the number of reactions of PCR can be made fewer than that of a conventional VNTR method, and the number of lanes required for electrophoresis can also be lessened, and the typing method is useful as a method of checking a herd infection misgiving case of a tubercle bacillus very easily and certainly. <P>COPYRIGHT: (C)2012,JPO&INPIT |
