| 发明名称 |
Process for the production of cellulase-amylase complex |
| 摘要 |
The invention relates to biotechnology, in particular to the production of a cellulase-amylase complex which can be used in animal husbandry as a means to improve the assimilation of food rations.The process for the production of cellulase-amylase complex includes submerged cultivation of Aspergillus niger CNMN-FD-10 fungus strain on a nutrient medium in the following component ratio, g/L: sugar beet pulp - 10.0; starch - 10.0; wheat bran - 20.0 ; KH2PO4 - 1.0; CaCl2·2H2O - 0.1; MgSO4·7H2O - 0.3; NaNO3 - 2.5; water up to 1.0 L, at pH 6.0, during 168 hours, after cultivation the culture liquid is separated from the biomass by filtration or centrifugation, the cellulase-amylase complex is separated from the culture filtrate by sedimentation with rectified ethyl alcohol and centrifuged. |
| 申请公布号 |
MD4121(B1) |
申请公布日期 |
2011.07.31 |
| 申请号 |
MD20100000101 |
申请日期 |
2010.09.16 |
| 申请人 |
INSTITUTUL DE MICROBIOLOGIE SI BIOTEHNOLOGIE AL ACADEMIEI DE STIINTE A MOLDOVEI;INTREPRINDEREA DE STAT PENTRU CERCETARE IN SELECTIA SI HIBRIDAREA SUINELOR "MOLDSUINHIBRID" A INSTITUTULUI STIINTIFICO-PRACTIC DE BIOTEHNOLOGII IN ZOOTEHNIE SI MEDICINA VETERINARA AL ASM;UNIVERSITATYA AGRAREH DE STAT DIN MOLDOVA;INSTITUTUL DE MICROBIOLOGIE ⍼I BIOTEHNOLOGIE AL ACADEMIEI DE ⍼TIIN⍼E A MOLDOVEI;INTREPRINDEREA DE STAT PENTRU CERCETARE IN SELEC⍼IA ⍼I HIBRIDAREA SUINELOR "MOLDSUINHIBRID" A INSTITUTULUI ⍼TIIN⍼IFICO-PRACTIC DE BIOTEHNOLOGII IN ZOOTEHNIE ⍼I MEDICIN⍼ VETERINAR⍼ AL A⍼M;UNIVERSITATEA AGRAR⍼ DE STAT DIN MOLDOVA |
发明人 |
DESYATNIK-CHILOCHI ALEXANDRA;TIURINA JANETTA;KLAPKO STELIANA;CAISIN LARISA;HAREA VASILE;LABLYUK SVETLANA;STRATAN MARIA;DVORNINA ELENA;DESEATNIC-CILOCI ALEXANDRA;CLAPCO STELIANA;LABLIUC SVETLANA |
| 分类号 |
C12N1/14;A23K1/165;C12N9/26;C12N9/28;C12N9/42;C12N9/62;C12R1/685 |
主分类号 |
C12N1/14 |
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