| 摘要 |
This invention relates to a method for detecting an analyte in a sample. The method comprises the steps of exposing the sample to a transducer having a pyroelectric or piezoelectric element and electrodes which is capable of transducing a change in energy to an electrical signal, the transducer having at least one reagent proximal thereto, the reagent having a binding site which is capable of binding the analyte or a complex or derivative of the analyte, wherein at least one of the analyte or the complex or derivative of the analyte has a label attached thereto which is capable of absorbing the electromagnetic radiation generated by the radiation source to generate energy by non-radiative decay; irradiating the reagent with a series of pulses of electromagnetic radiation, transducing the energy generated into an electrical signal, detecting the electrical signal and the time delay between each pulse of electromagnetic radiation from the radiation source and the generation of the electric signal. The time delay between each of the pulses of electromagnetic radiation and the generation of the electric signal corresponds to the position of the analyte at any of one or more positions at different distances from the surface of the transducer. The label is a nanoparticle comprising polypyrrole or a derivative thereof. The invention also provides a kit suitable for performing this method.
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