发明名称 |
FLUOROIMMUNOASSSAY METHOD |
摘要 |
Disclosed is an immunoassay method which does not require a solid phase immobilization step and a washing step, enables the rapid, simple and quantitative measurement of a substance of interest in a liquid phase, and enables the visualization of an antigen. The concentration of an antigen of interest in a substance to be tested can be measured by carrying out the followings steps (a) to (c) sequentially: (a) bringing an antibody light-chain variable region polypeptide and an antibody heavy-chain variable region polypeptide labeled with a fluorescent dye into contact with the antigen in the substance to be tested in a liquid phase, or bringing an antibody heavy-chain variable region polypeptide and an antibody light-chain variable region polypeptide labeled with a fluorescent dye into contact with the antigen in the substance to be tested in a liquid phase; (b) measuring the fluorescence intensity of the fluorescent dye; and (c) calculating the amount of the antigen contained in the substance to be tested on the basis of the positive correlation between the concentration of the antigen in the liquid phase and the fluorescence intensity of the fluorescent dye. |
申请公布号 |
CA2780845(A1) |
申请公布日期 |
2011.05.26 |
申请号 |
CA20102780845 |
申请日期 |
2010.11.19 |
申请人 |
PROTEINEXPRESS CO., LTD. |
发明人 |
UEDA, HIROSHI;ABE, RYOJI;IHARA, MASAKI;TAKAGI, HIROAKI |
分类号 |
G01N33/533;C07K16/18;G01N21/78;G01N33/531 |
主分类号 |
G01N33/533 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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