Module-based vector useful in the assessment of antigen-specific T cell functions

摘要

The present invention refers to a module-based vector for concomitant eukaryotic expression of a proteasome degradable antigenic first protein and an accessory second protein, preferably a fluorescent reporter protein or a membrane-bound co-stimulatory protein. In particular, the module-based vector comprises a first nucleic acid sequence encoding the proteasome-degradable protein comprising a section encoding ubiquitin upstream of a section encoding the antigenic first protein, a second nucleic acid sequence encoding the accessory second protein, and a third nucleic acid sequence for encoupling the antigenic first and the accessory second protein which is located between the first and the second nucleic acid sequence. The present invention also refers to a use of the module-based vector for assessing antigen-specific T cell functions. The present invention further refers to a plasmid transfection fluorolysis (PFT) assay using the module-based vector.