| 摘要 |
The invention relates to a method for synthesizing a nucleic acid containing modified nucleotides. The method encompasses the following steps: a matrix strand is provided;—a primer which at least partially hybridizes on the matrix strand is provided;—nucleoside triphosphates, at least some of which are modified nucleoside triphosphates, are provided;—a polymerase activity is supplied; and—the matrix strand, the primer, and the nucleoside triphosphates are incubated so as to synthesize a nucleic acid that is substantially complementary to the matrix strand. The polymerase activity can be a reverse transcriptase activity.
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