| 摘要 |
PROBLEM TO BE SOLVED: To provide a class of rhodamine dyes having fluorescence spectra which are substantially narrower than those of presently available rhodamine dyes, and to provide a class of rhodamine dyes having absorption spectra shifted to the red as compared to existing rhodamine dyes.SOLUTION: A set of 4,7-dichlororhodamine compounds useful as fluorescent dyes is disclosed. In another aspect, the invention includes reagents labeled with the 4,7- dichlororhodamine dye compounds, including deoxynucleotides, dideoxynucleotides, and polynucleotides. In an additional aspect, the invention includes methods utilizing such dye compounds and reagents, including dideoxy polynucleotide sequencing and fragment analysis methods. |
