发明名称 |
METHOD FOR DETERMINING THE SENSITIVITY OR RESISTANCE OF RETROVIRUS ISOLATES TO MOLECULES, THERAPEUTIC RETROVIRAL TREATMENTS BASED ON VIRAL PROTEASE INHIBITORS AND DIAGNOSTIC KITS |
摘要 |
A method of determining sensitivity or resistance of isolates of HIV retroviruses to a molecule includes a) amplifying sequences coding for a protease of a retrovirus to be studied, with or without the or some of amino acid sequences situated upstream and downstream of a cleavage site of a precursor in which the amino acid sequences are situated, b) recombining fragments of DNA, a final product of the amplification, and an expression vector allowing expression of sequence coding for the protease of the retrovirus to be studied under control of a known inducible promoter through co- transformation of the vector and the DNA fragments with at least one yeast cell, c) culturing co-transformed yeast cell or cells to obtain a sufficient number of transformants to perform a sensitivity or resistance test, and recovering transformants issuing from the co-transformed cell, on any suitable medium, d) incubating the transformants in the presence of a molecule to be tested, e) qualitatively or quantitatively analyzing the living cells, and f) deducing the sensitivity or resistance phenotype. |
申请公布号 |
WO2011007244(A1) |
申请公布日期 |
2011.01.20 |
申请号 |
WO2010IB01738 |
申请日期 |
2010.07.14 |
申请人 |
UNIVERSITE DE LA MEDITERRANEE;CNRS;GLUSCHANKOF, PABLO;RAOULT, DIDIER;BEN M'BAREK, NAJOUA;AUDOLY, GILLES;PERRIN-EAST, CHRISTELLE |
发明人 |
GLUSCHANKOF, PABLO;RAOULT, DIDIER;BEN M'BAREK, NAJOUA;AUDOLY, GILLES;PERRIN-EAST, CHRISTELLE |
分类号 |
C12N15/10;C12Q1/70 |
主分类号 |
C12N15/10 |
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