Nanostructured surfaces as a dual ionization LDI-DESI platform for increased peptide coverage in proteomic analysis

摘要

Embodiments of the invention include methods and devices for the analysis of proteins utilizing a gold coated nanoporous alumina surface for dual ionization mode mass spectrometric analysis using desorption electrospray ionization (DESI) and laser desorption ionization (LDI). Combined use of DESI and LDI gives increased sequence coverage in peptide mixture analysis from a single sample preparation.