摘要 |
<p>The purpose of the present invention is to develop a pretreatment technique which enables, as a pretreatment for enzymatically saccharifying a starting material for a lignocellulose-based biomass (including a starting material for a lignocellulose-based biomass containing easily degradable saccharides), the performance of efficient saccharification without causing outflow of saccharides (in particular, free saccharides, starch, xylan, and the like) due to solid/liquid separation or washing steps. Disclosed are: a method for producing a slurry to be used as a substrate in enzymatic saccharification, which comprises grinding the above-ground parts of a starting plant material for a lignocellulose-based biomass, preparing a slurry containing said starting material, calcium hydroxide and water, treating the slurry with an alkali, and then neutralizing the same by bubbling carbon dioxide gas thereinto and/or pressurizing to decrease the pH of the slurry to 5-7; an enzymatic saccharification method using, as a substrate, a slurry obtained by said method for producing a slurry; and an ethanol fermentation method using, as a substrate, a saccharified product obtained by said enzymatic saccharification method.</p> |