摘要 |
The present invention relates to in vitro adhesion modules that allow growth, stabilization and study of microbial communities that adhere to and colonize host- related surfaces, that mimic transport of chemical compounds across epithelial surfaces and simulate host-microorganism interactions and adaptation. It includes the provision of micromolar amounts of oxygen via the basal side of a mucus layer towards the adhered microorganisms thus establishing the microaerophilic conditions prevailing at the base of a biof?lm. It can also include cells, simulating the host, in a chamber on the basal side of a functional layer comprising said mucus layer. The adhesion module of the present invention can be placed between the different compartments of the SHIME - the Simulator of the Human Intestinal Microbial Ecosystem. An extension of the SHIME is made where the duodenum, jejunum and ileum are separately mimicked. |
申请人 |
UNIVERSITEIT GENT;MARZORATI, MASSIMO;POSSEMIERS, SAM;VAN DEN ABBEELE, PIETER;VAN DE WIELE, TOM;VANHOECKE, BARBARA;VERSTRAETE, WILLY |
发明人 |
MARZORATI, MASSIMO;POSSEMIERS, SAM;VAN DEN ABBEELE, PIETER;VAN DE WIELE, TOM;VANHOECKE, BARBARA;VERSTRAETE, WILLY |