摘要 |
A method of identifying a compound that modulates NCKX-mediated calcium ion exchange across a cell membrane, the method comprising the steps of providing an adherent cell, the cell membrane of which comprises a NCKX polypeptide or a functionally equivalent variant thereof, exposing the cell in suspension to a fluorescent calcium-sensitive dye, thereby causing intracellular uptake of the dye, settling the cell onto a solid support without allowing the cell to adhere to the solid support, exposing the cell to a test compound, exposing the cell to calcium and potassium ions, and measuring fluorescence from the intracellular calcium-sensitive dye after exposure of the cell to the test compound and the calcium and potassium ions, thereby to determine the rate and/or amount of calcium ion exchange across the cell membrane in the presence of the test compound. |
申请人 |
UNILEVER PLC, A COMPANY REGISTERED IN ENGLAND ANDWALES UNDER COMPANY NO. 41424 OF UNILEVER HOUSE;UNILEVER N.V. |
发明人 |
OGBORNE, RICHARD, MARTIN;SCHNETKAMP, PAULUS, PETRUS, MARIUS |