发明名称 |
Reversed mammalian protein-protein interaction trap |
摘要 |
The invention relates to a recombinant receptor, comprising a ligand-binding domain and a signaling domain that comprises a heterologous bait polypeptide, which receptor is inactivated by binding of a prey polypeptide to the heterologous bait peptide, either in presence or absence of a ligand binding to the ligand-binding domain. The receptor is activated by addition of a compound that disrupts the bait-prey interaction. The invention also relates to a method of screening compounds that disrupt compound-compound binding using the recombinant receptor.
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申请公布号 |
US2010174049(A1) |
申请公布日期 |
2010.07.08 |
申请号 |
US20090653513 |
申请日期 |
2009.12.14 |
申请人 |
VLAAMS INTERUNIVERSITAIR INSTITUUT VOOR BIOTECHNOLOGIE VZW |
发明人 |
EYCKERMAN SVEN;TAVERNIER JAN;VANDKERCKHOVE JOEL |
分类号 |
C07K14/00;C07K14/47;C07K14/705;C12N15/10 |
主分类号 |
C07K14/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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