Electrosensing antibody-probe detection and measurement sensor and method

摘要

A sensor (400) and its method for electrosensing an antigen (432) in a test sample are disclosed. The sensor (400) has two electrodes (412, 414) electrically disconnected and physically separated from each other, and a layer of antibody (422) is immobilized on the surface of at least one of the electrodes (412, 414). The antibody (422) has specific binding reactivity with the antigen (432). Conductivity promotion molecules (442) may be tethered over and/or distributed between the antibody-populated electrodes (412, 414) for improving electrical conductivity characteristics across the two electrodes. The antibody (422) captures the antigen (432) present in the test sample mixed in a buffer solution that comes into contact with the antibody-populated electrodes. This alters the electrical conductivity characteristic across the two electrodes (412, 414) in which an amount representative of the altering provides an indication for electrosensing of the antigen (432).