发明名称 |
METHOD FOR PURIFICATION OF NUCLEIC ACIDS, PARTICULARLY FROM FIXED TISSUE |
摘要 |
The invention relates to a method for purification of nucleic acids, to a kit for performing the method according to the invention and to a new application of magnetic particles for purification of a biological sample. The method according to the invention comprises the following steps: a) accommodating of the sample in a first sample vessel in an aqueous solution and lysing of the sample under non-chaotropic conditions; suspending of first magnetic particles in the solution and inserting of the first sample vessel in a sample vessel holder, wherein the sample vessel is inserted in the annular interior space of a ring magnet associated with the sample vessel holder; separating of the solution from the magnetic particles; and isolating of the nucleic acids from the solution. |
申请公布号 |
CA2746489(A1) |
申请公布日期 |
2010.06.17 |
申请号 |
CA20092746489 |
申请日期 |
2009.11.20 |
申请人 |
SIEMENS HEALTHCARE DIAGNOSTICS INC. |
发明人 |
EUTING, HEIKE;HENNIG, GUIDO;IZMAILOV, ALEXANDRE |
分类号 |
C12N15/10 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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