| 摘要 |
<p>DNA molecule for expressing hairpin RNA, the constructing method and the use thereof are provided. The DNA molecule is a closed circular single-stranded DNA molecule which is constructed by linking four fragments A,B,C and D as following order: A-C-B-D or A-D-B-C, wherein A is a sense strand or anti-sense strand fragment of the target double-stranded DNA; B is the fragment which is complementary to A; C and D are DNA fragments with stem and loop structure and they meet one of the following requirements: 1) the nucleotide sequence of fragment C or D comprises at least one sequence e; 2)the nucleotide sequence of fragment C comprises at least one sequence e and the nucleotide sequence of fragment D comprises at least one sequence f, wherein sequences e and f individually are one strand of different restriction endonuclease recognition sequences. The DNA molecule for expressing hairpin RNA and its constructing method are useful in constructing lhRNA library.</p> |
