发明名称 |
Systematic identification of new anti-prion drugs by high-throughput screening based on scanning for intensely fluorescent targets (sift) |
摘要 |
The present invention relates to a method of identifying a compound for inhibiting aggregation of proteins involved in diseases linked to protein aggregation and/or neurodegenerative diseases, comprising the steps of: (a) bringing into contact a labeled monomeric protein and a differently labeled aggregate of said protein in the (1) presence and (2) absence of a candidate inhibitor of aggregation, (b) determining the amount of co-localized labels, representing the extent of binding of the monomeric proteins to the aggregates of said protein; and (c) comparing the result obtained in the presence and absence of said compound, wherein a decrease of co-localized labels in the presence of said compound is indicative of the compound's ability to inhibit aggregation of said protein. Moreover, the present invention relates to a pharmaceutical composition containing said inhibitor of aggregation as well as to a kit.
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申请公布号 |
US2010029773(A1) |
申请公布日期 |
2010.02.04 |
申请号 |
US20090597672 |
申请日期 |
2009.09.28 |
申请人 |
LUDWIG-MAXIMILIANS-UNIVERSITAT MUNCHEN;MAX-PLANCK-GESELLSCHAFT FUR FORDERUNG DER WISSENSCHAFTEN E.V. |
发明人 |
BERTSCH UWE;GIESE ARMIN;KRETZSCHMAR HANS;TAVAN PAUL;HIRSCHBERGER THOMAS;BIESCHKE JAN;WEBER PETRA;WINKLHOFER KONSTANZE F.;TATZELT JOERG;HARTL ULRICH F.;MITTERREGGER-KRETZSCHMAR GERDA;HOEGEN TOBIAS JOHANNES |
分类号 |
A61K31/16;A61K31/155;C07C241/00;C07C281/00;C12Q1/02;G01N33/48;G01N33/53;G01N33/566;G01N33/68 |
主分类号 |
A61K31/16 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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