| 摘要 |
<p>Methods, devices, and systems for measuring the concentration of glycated hemoglobin (HbA1c) in a blood sample are provided. A blood sample may be contacted with a lysing agent, an assay to measure total Hb, and an assay to measure HbA1c, which comprises donor and acceptor dyes attached to a Hb specific antibody and a HbA1c analog. Preferably, the emission spectrum of the donor dye overlaps with the excitation spectrum of the acceptor dye. The concentration of HbA1c in the sample may be correlated with an increase in donor dye and/or a decrease in acceptor dye fluorescence in the presence of HbA1c as compared to the donor and/or the acceptor dye fluorescence in the absence of HbA1c.</p> |
