发明名称 |
IMMUNOASSAY FOR QUANTIFICATION OF AN UNSTABLE ANTIGEN SELECTED FROM BNP AND PROBNP |
摘要 |
The present invention relates to an immunoassay for detection of BNP, proBNP and fragments thereof. Essentially the assay comprises: a) contacting the antigen with a first antibody specific to a fragment corresponding to amino acids 11-22 of BNP, or to a part of this peptide comprising at least three amino acids of said sequence, to obtain a first order immune complex. b) contacting the first order immune complex obtained at step (a) with a second antibody recognizing said first order immune complex, to obtain a second order immune complex, wherein said antibody is unable to recognize free BNP, proBNP or free first antibody; c) Detecting the second order immune complex. |
申请公布号 |
EP2135087(A1) |
申请公布日期 |
2009.12.23 |
申请号 |
EP20080736833 |
申请日期 |
2008.04.14 |
申请人 |
HYTEST LTD. |
发明人 |
TAMM, NATALIA, N.;KATRUKHA, ALEXEY, G.;FILATOV, VLADIMIR, L.;KOLOSOVA, OLGA, V. |
分类号 |
G01N33/74;C07K16/26;G01N33/577;G01N33/68 |
主分类号 |
G01N33/74 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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