METHOD OF DETERMINING THE SPATIAL CONFIGURATION OF MOLECULES IN PARTICLES OR MACROMOLECULES, ESPECIALLY FOR DETERMINING THE SHAPE OF METAL NANOPARTICLES AND DEVICE FOR THE IMPLEMENTATION THEREOF

摘要

The present invention relates to a method and a device for determining the spatial configuration of molecules in particles or macromolecules or the shape of metal nanoparticles. This method provides for the excitation of said particles or macromolecules placed in solution by means of two identical pulsed laser beams (E1, E2) but having different angles of incidence I1, I2 relative to the particles tested, followed by the detection of the SHG (second harmonic generation) light photons and establishment of the polarization-resolved HRS (hyper-Rayleigh scattering) intensity plots for each of the excitation beams. From each HRS plot thus determined, a parameter ?E1, ?E2 characteristic of the spatial configuration of the molecules within the tested particles is calculated for each beam, which is determined relative to the parameters calculated in a preconstructed ?E2 = f(?E1) plot.