| 摘要 |
PURPOSE: A method for simultaneously diagnosing three different etiologic agents causing porcine berlock dermatitis, porcine swine dysentery and porcine Salmonella enteritis is provided, thereby the diagnosis time can be shortened, and the diagnosis can be carried out by using feces of pig, so that early detection and prevention of the diseases can be accomplished. CONSTITUTION: The method for simultaneously diagnosing etiologic agents comprises the steps of: preparing three primers having different size of Lawsonia intracellularis, Serpulina hyodysenteriae, and salmonella causing porcine berlock dermatitis, porcine swine dysentery and porcine Salmonella enteritis, respectively; mixing DNA isolated from feces of pig, polymerase and buffer solution; denaturing the DNA by heating to separate double stranded DNA into single stranded DNA; annealing the primer with the single stranded DNA; amplifying the specific gene of the microorganisms by extending the primer with polymerase; and detecting the specific gene of microorganisms relating to diseases by ultraviolet irradiation.
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